The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products ended up digested and cloned right into a pET21a+ vector making use of NdeI and XhoI restriction enzymes. Some extent mutation was released by https://davidy211kvg3.qodsblog.com/profile